RNA preparation reagents

[tabrams]

There are several protocols and commercial reagents available for RNA preparation. In conversations with researchers involved in the Aplysia Genome at the Broad Institute, it seems they have found Trizol adequate for RNAseq studies of most other species. Does anyone have experience that would suggest we should compare other RNA preparation reagents?

[Sathya]

I have prepared RNAs using multiple methods and analyzed by Northerns, RNAseq, PCR, making full length libraries. Ambion kits, standard good old Guanidinium method and trizol were used depending on the amount of tissues I am using for specific experiments. For my downstream applications (see above), these methods did not make any difference. I must admit that for preparing the full length libraries I used Guanidinium and Trizol methods. I would go with Trizol method. Probably we should process the samples immediately after isolating from the animals.